2006•Biotechnology(Faisalabad)Requires access
Expression of HBV PreS2-MBP Fusion Protein in E.coli Different Part
Jin Lei
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Abstract
Objectives:To analyze the expression of HBV PreS2 protein in E.coli different part.Methods:Recombinant plasmids pMAL-P2x/S2 and pMAL-C2x/S2 were constructed for expressing HBV PreS2 fusion protein in E.coli periplasm and cytoplasm.The E.coli DH5α with the two recombinant plasmids were induced under IPTG.The expression products and their exist forms was identified by SDS-PAGE and Western Blot.Results:The degradation have been occurred on some of the fusion protein was expressed in E.coli periplasm.The total HBV PreS2-MBP fusion protein was expressed in E.coli cytoplasm.The fusion protein was expressed in cytoplasm have two exist forms,inclusion bodies and solubility protein.Conclusion: HBV PreS2-MBP fusion protein is fit to express in E.coli cytoplasm.