Determination of Microcystin-LR in Water by SPE and HPLC

The enrichment and detection of microcystin-LR were performed by the solid phase extraction(SPE) and high performance liquid chromatography(HPLC).The microcystin-LR was extracted with 80% aqueous methanol(m/V) stirring for 2 h at room temperature when the fluid solid ratio was 1 ∶ 50.The supernatant was obtained after centrifugation(20 min,8 000 r/min).The methanol in the extract was removed by the rotary evaporator,and the color was removed by the protein isoelectric point precipitation process.SPE was used for the microcystin-LR concentration and partial purification.The microcystin-LR was identified and quantified by HPLC.The method has good accuracy,precision,recovery rate,high extraction rate and low impurity content.The minimum detection limit was 0.1 mg/L.The method is suitable for analyzing the trace microcystin-LR in water.