2003•Unpublished venueRequires access

Expression of recombinant soluble human CD137 in CHO cells

Wensheng Sun

Abstract

Objective:To get CHO cell lines expressing soluble human CD137antigen.Methods:The recombinant CD137-hIgG1Fc-pCDNA3plasmid and pSV2-dhfr plasmid were co-transfected into dhfr-CHO cells by lipoid mediating method.The positive clones were selected by G418.Expression of soluble human CD137on dhfr-CHO cells was induced by MTX.Transcription and expression of CD137were detected by RT-PCR and SDS-PAGE.Results:A specific band of558bp for extracellular part of human CD137was detected by RT-PCR in CHO cells transfected by recombinant plasmid.A band of37KDs peptide,same to human CD137-hIgG1Fc fusion protein in size,was got by SDS-PAGE in culture supernant of transfected CHO cells.Conclusion:CHO cells which expressed soluble human CD137were got.This would be helpful for getting purified CD137antigen and antibody to CD137.

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Key concepts: Chinese hamster ovary cell, Recombinant DNA, Transfection, Molecular biology, CD137, Cell culture, Fusion protein, Plasmid