New acute pancreatitis rats model accompanied with extrapancreatic multi-organ injury

Objective: To explore a new severe but sublethal acute pancreatitis model for the study of clinically relevant extrapancreatic multiorgan injury. Methods: The animals were divided into two groups: the bile acid injection group and the control group. In the bile acid injection group, acute necrotizing pancreatitis was induced by intraductal administration of 0.2 ml of 2.0 % bile acid under 30 cm H2O pressure, while the controls underwent the sham operation. The two groups were divided into six subgroups (8 rats for each) and sacrificed at 12, 24, 36, 48, 72 and 144 hours, respectively. The structural and functional features in the pancreas, lung, liver and kidney were examined. Results: Accompanied with acute necrosis pancreatitis, the functional and histologic changes had developed continuously in the kidney and liver for 72 and 144 hours in the bile acid injection animals respectively. No pancreatitis associated pulmonary changes were found. Conclusions: The sublethal acute pancreatitis model is suited for pathogenetic and therapeutic study of liver and renal changes in acute necrotizing pancreatitis.