Effect of sulphur on the content of GSH and the activity of GSH-PX in wheat leave under drought stress

Glutathione(GSH) in organism may play an important role on elimination of reactive Oxygen species produced under stress.There are close relationship between absorbation of inorganic sulphur and transformation/assimilation of organic sulphur in organism.Research on effect of sulphur on the content of GSH and the activity of gutathione peroxidase(GSH-PX) in wheat leave under drought stress may have a greatly significance for illustrating resisting stress mechanism of sulphur nutrition.The content of GSH and the activity of GSH-PX of the seedling of drought-sensitive wheat strain Zhengyin No.1 and drought-tolerant strain Shanhe No.6 cultured in complete Hoagland solution containing sulphur and Hoagland solution without sulphur were compared.The results showed the content of GSH of drought-tolerant wheat was clearly higher than the drought-sensitive wheat.Under drought stress,the GSH content in Zhengyin No.1 with the sulphur-lack treatment were rised rapidly,in which increasing 26.4%,33.5% and 56.6% compare to Shanhe No.6,at 24 h,48 h and 72 h of drought stress,respectively.For Shanhe No.6,the chenges of GSH content during 72 h drought stress with the treatment of sulphur-rich were unconspicuous,which was 98.3% to 106.1% under providing water,but the one with the treatment of sulphur-lack rised conspicuously,was 13.1%,40.2% and 45.9% than under providing water.The GSH content for Zhengyin No.1 was remarkable impressed by drought stress and suppplyment of sulphur nutrition,but for Shanhe No.6,was only sensitively to drought stress under the sulphur-lack.At the initial stages(24 h),the GSH-PX activities were increased. For Zhengyin No.1,GSH-PX activities under the treatment of sulphur-rich were 23.9% higher than under the water-providing treatment,but it was only 7.4% under the treatment of sulphur-lack higher than the treatment of water-providing.;for Shanhe No.6,GSH-PX activities were respectively 24.5% under the treatment of sulphur-rich and 16.8% under the treatment of sulphur-lack higher than the treatment of water-providing,respectively.As the prolong of the drought stress,the activities of GSH-PX were all fall down,but under the treatment of sulphur-rich it was always higher than the treatment of sulphur-lack.For Zhengyin No.1,the activities of GSH-PX level were lower,and unsensitively to drought stress under the sulphur-lack,however,for Shanhe No.6,it was all higher,and sensitively to drought stress.