Effects of Different Bacterial Culture Condition on the Expression of Avian Salmonella Fimbrial Genes

The objective of the present study was to investigate the distribution of fimbrial genes in some avian Salmonella serotypes and the expression differences of fimbrial genes under different bacterial culture conditions.Seventeen kinds of fimbrial operon genes in Salmonella pullorum,Salmonella gallinarum,Salmonella typhimurium and Salmonella enteritidis were detected by PCR,and expression of fimbrial genes in the above Salmonella strains under three different culture conditions which include static broth,shaken broth and static plate were detected by RTPCR.Results showed that the four Salmonella serotypes had different fimbrial genotypes,10 of which were conservative,and the distribution of the other 7 fimbrial genes was different.RT-PCR detection results showed that the expression of fimbrial genes in different serotypes cultured under three conditions had some differences,as the expression of fimbrial genes in Salmonella pullorum and Salmonella enteritidis cultured on static broth was more than that of others(P0.05),while the difference in Salmonella gallinarum and Salmonella ty phimurium was not significant(P0.05).The results revealed that the four Salmonella serotypes had different fimbrial genotypes and the bacterial culture condition significantly impacted the expression of fimbrial genes in Salmonella pullorum and Salmonella enteritidis.