Effects of Ultraviolet Irradiation on the Development of Parthenogenic and in Vitro Fertilization Embryos in Bovine

Effects of different time ultraviolet(UV) irradiation combined with fluorescence Hoechst33342 on the cleavage and blastocyst development of parthenogenic and in vitro fertilization embryos in bovine were investigated. The results showed that the cleavage and blastocyst development of parthenogenic embryos derived from oocytes irradiated with UV 30s and 40s were significantly lower than those of oocytes irradiated with UV 0 s, 10 s and 20,s ( P 0.01). It indicated that irradiating oocytes with UV longer than 20s decreased the developmental potential of parthenogenic embryos significantly.In in vitro fertilization research, the cleavage rate of embryos derived from the irradiated oocytes suffered from UV irradiation 30 s and 40 s are significantly lower than that suffered from UV irradiation 10 s and 20 s ( P 0.05),furthermore,the blastocyst development rate had significant difference between 20s and 10s.The results indicated that irradiating oocytes with UV no longer than 20s could obtain the cleavage and blastocyst developmental potential of bovine embryos from parthenogenic and in vitro fertilization.