Screening and characterization of a bacteriocin-producing strain from the Chinese pickles

One strain of bacteriocin production bacterium was isolated from the pickles water,detecting its appearance,physiological and biochemical characteristics,it can be identified as a lactic acid bacterium.Through the sensitivity of rough bacteriocin to temperature,pH and protease,the results showed that the supernatant of the strain was sensitive with pepsin and papain,the inhibitory material of the strain was a kind of protein,which could be classed as bacteriocin.The bacteriocin had the strong heat endurance(121 ℃,20 min) and strong bacteriostatic activity(pH 3.0～5.0).The bacteriocin showed inhibition to some gram-positive and gram-negative bacteria.Therefore,it was a kind of bacteriocin with broad-spectrum antimicrobial activity.