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Callus induction from anthers of Paulownia tomentosa× Paulownia fortunei

Guoqiang Fan

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Abstract

Callus induction from anthers of Paulownia tomentosa× Paulownia fortune was studied in this paper.The results showed that it was an ideal disinfection way to soak explants in 70% alcohol for 30 s first and then in 0.1% HgCl2 for 60 s.The rate of callus induction at 4 ℃ temperature for 7 days can reached the highest.The 3% sucrose concentration was suitable for the induction,and the rate of induction reached 58.76%.The best medium to induce calli from anthers is MS medium + 30 g·L-1 sucrose + 2 mg·L-1NAA + 3 mg·L-1 6-BA.

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What this paper is about

Callus induction from anthers of Paulownia tomentosa× Paulownia fortune was studied in this paper.The results showed that it was an ideal disinfection way to soak explants in 70% alcohol for 30 s first and then in 0.1% HgCl2 for 60 s.The rate of callus induction at 4 ℃ temperature for 7 days can reached the highest.The 3% sucrose concentration was suitable for the induction,and the rate of induction reached 58.76%.The best medium to induce calli from anthers is MS medium + 30 g·L-1 sucrose + 2 mg·L-1NAA + 3 mg·L-1 6-BA.

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Available abstract

Callus induction from anthers of Paulownia tomentosa× Paulownia fortune was studied in this paper.The results showed that it was an ideal disinfection way to soak explants in 70% alcohol for 30 s first and then in 0.1% HgCl2 for 60 s.The rate of callus induction at 4 ℃ temperature for 7 days can reached the highest.The 3% sucrose concentration was suitable for the induction,and the rate of induction reached 58.76%.The best medium to induce calli from anthers is MS medium + 30 g·L-1 sucrose + 2 mg·L-1NAA + 3 mg·L-1 6-BA.

Key concepts: Paulownia, Callus, Stamen, Sucrose, Botany, Explant culture, Biology, Horticulture

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