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A preliminary study on superovulation and the embryos of Wistar rats at different developmental stages

Mu Liu

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Abstract

To obtain the methods for embryo collection and the optimal dose of the reagents for superovulation to be used in Wistar rats at different developmental stages,eight to nine-week-old Wistar rats were superovulated with 10 IU,20 IU and 30 IU of pregnant mare serum gonadotropin-human chorionic gonadotropin(PMSG-HCG),respectively.The results showed that the number of two-cell embryos obtained from the three groups were 12.1±4.6,23.1±10.2 and 24.9±8.2 respectively,in which the superovulation with 30 IU was considered to be the best one.The numbers of morula obtained from the three groups were 10.3±5.0,23.7±17.2 and 17.2±5.9 respectively,in which the superovulation with 20 IU was considered to be the best one.

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What this paper is about

To obtain the methods for embryo collection and the optimal dose of the reagents for superovulation to be used in Wistar rats at different developmental stages,eight to nine-week-old Wistar rats were superovulated with 10 IU,20 IU and 30 IU of pregnant mare serum gonadotropin-human chorionic gonadotropin(PMSG-HCG),respectively.The results showed that the number of two-cell embryos obtained from the three groups were 12.1±4.6,23.1±10.2 and 24.9±8.2 respectively,in which the superovulation with 30 IU was considered to be the best one.The numbers of morula obtained from the three groups were 10.3±5.0,23.7±17.2 and 17.2±5.9 respectively,in which the superovulation with 20 IU was considered to be the best one.

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Available abstract

To obtain the methods for embryo collection and the optimal dose of the reagents for superovulation to be used in Wistar rats at different developmental stages,eight to nine-week-old Wistar rats were superovulated with 10 IU,20 IU and 30 IU of pregnant mare serum gonadotropin-human chorionic gonadotropin(PMSG-HCG),respectively.The results showed that the number of two-cell embryos obtained from the three groups were 12.1±4.6,23.1±10.2 and 24.9±8.2 respectively,in which the superovulation with 30 IU was considered to be the best one.The numbers of morula obtained from the three groups were 10.3±5.0,23.7±17.2 and 17.2±5.9 respectively,in which the superovulation with 20 IU was considered to be the best one.

Key concepts: Human chorionic gonadotropin, Equine chorionic gonadotropin, Embryo, Biology, Andrology, Gonadotropin, Endocrinology, Internal medicine

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A preliminary study on superovulation and the embryos of Wistar rats at different developmental stages — Research Paper | ScholarLens