Optimization of Microwave-assisted Extraction of Flavonoids of Green Tea Using Response Surface Analysis
Hairong Zhang
Abstract
Hairong Zhang
Abstract
Response surface methodology with three factors and three levels was used to optimize the microwave-assisted extraction of flavonoids of green tea,on the basis of a series of one-factor experiments,second linear regression equation was obtained with flavonoids yield as a response surface value by the Design-expert software,the optimum extraction conditions of flavonoids of green tea were determined as follows: 600 W of extraction power,3.5 min of extraction time,and 50 ∶ 1(mL/g) of liquid-to-solid ratio,and twice of extraction times,the extraction efficiency of flavonoids was up to 2.605 mg/g under the optimized conditions.Confirmative test indicated that the established model had good prediction ability.
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Response surface methodology with three factors and three levels was used to optimize the microwave-assisted extraction of flavonoids of green tea,on the basis of a series of one-factor experiments,second linear regression equation was obtained with flavonoids yield as a response surface value by the Design-expert software,the optimum extraction conditions of flavonoids of green tea were determined as follows: 600 W of extraction power,3.5 min of extraction time,and 50 ∶ 1(mL/g) of liquid-to-solid ratio,and twice of extraction times,the extraction efficiency of flavonoids was up to 2.605 mg/g under the optimized conditions.Confirmative test indicated that the established model had good prediction ability.
Key concepts: Extraction (chemistry), Response surface methodology, Yield (engineering), Green tea, Chromatography, Microwave power, Design–Expert, Chemistry