influence of high glucose-induced stress on the survival of H9c2 cells

Objective To investigate the potential influence of high glucose on the survival of H9c2 cells and posible signaling pathways.Methods After the H9c2 cells were cultured for 24 hours in the high glucose condition,the cells were collected for determining the cell viability and apoptosis.The phosphorylation of Akt and expression of eNOS were measured by Western blot.Results High glucose improved the viability of H9c2 cells and reduced their apoptosis.High glucose increased the phosphorylation of Akt followed by increased eNOS expression.The PI3K inhibitor LY294002 abolished the protective effect of high glucose on H9c2 cells as well as the NOS inhibitor L-NAME.Conclusion In the early phase,high glucose protects the H9c2 cells from apoptosis through activating PI3K/Akt/eNOS signaling pathway.