Semi-quantitative detection of MDR gene mRNA expression by using multiple RT-PCR

Objective To establish multiple RT PCR semi quantitative detection system to test MDR gene mRNA expression.Methods Using duplicate RT PCR as method through a process of amplification to become a multi component RT PCR.Based on observation of 5 MDR gene's mRNA kinetic effect studies and the setting of RT PCR standard curves, a six complex RT PCR method was established.Results Six complex RT PCR system was identical to simple and duplicated RT PCR in sensitivity and specificity, and easily to be repeated.Conclusion This given method was sensitive, specific, and had the advantages of speed in performance (within 6 h),only small quantity of specimen (50 mg) and semi quantitation when used to detect the MDR gene mRNA expression in primary liver cancer tissue and cell line. It could also be applied to process a batch of samples simultaneously in one setting.