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Extraction and primary investigation of antitumor activity of polysaccharides from the volva of Dictyophora rubrovolvata

Wang Fei

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Abstract

The polysaccharides from the volva of Dictyophora rubrovolvata were extracted by hot water, precipitated by alcohol in different concentrations and deproteinized by the method of Sevag. The yield of crude polysaccharides of the volva were much higher than that of mycelium and other parts of fruit body. Two kinds of polysaccharides, DRVP1 and DRVP2, were purified by use of column chromatography on DEAE-cellulose and Sephadex G-75, and the structural features of the polysaccharides were elucidated by HPLC and IR respectively.The inhibitory effect of DRVP1 on the tumor cells of S180 (Sorcoma-180) was investigated by sulforhodamine-B (SRB) methods. The result showed that the molecular weight of DRVP1 was 1.47×104, and it had an inhibitory effect on the tumor cells of S180 to some extent.

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What this paper is about

The polysaccharides from the volva of Dictyophora rubrovolvata were extracted by hot water, precipitated by alcohol in different concentrations and deproteinized by the method of Sevag. The yield of crude polysaccharides of the volva were much higher than that of mycelium and other parts of fruit body. Two kinds of polysaccharides, DRVP1 and DRVP2, were purified by use of column chromatography on DEAE-cellulose and Sephadex G-75, and the structural features of the polysaccharides were elucidated by HPLC and IR respectively.The inhibitory effect of DRVP1 on the tumor cells of S180 (Sorcoma-180) was investigated by sulforhodamine-B (SRB) methods. The result showed that the molecular weight of DRVP1 was 1.47×104, and it had an inhibitory effect on the tumor cells of S180 to some extent.

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Available abstract

The polysaccharides from the volva of Dictyophora rubrovolvata were extracted by hot water, precipitated by alcohol in different concentrations and deproteinized by the method of Sevag. The yield of crude polysaccharides of the volva were much higher than that of mycelium and other parts of fruit body. Two kinds of polysaccharides, DRVP1 and DRVP2, were purified by use of column chromatography on DEAE-cellulose and Sephadex G-75, and the structural features of the polysaccharides were elucidated by HPLC and IR respectively.The inhibitory effect of DRVP1 on the tumor cells of S180 (Sorcoma-180) was investigated by sulforhodamine-B (SRB) methods. The result showed that the molecular weight of DRVP1 was 1.47×104, and it had an inhibitory effect on the tumor cells of S180 to some extent.

Key concepts: Polysaccharide, Chemistry, Sephadex, Chromatography, Column chromatography, Extraction (chemistry), Yield (engineering), Hot water extraction

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Extraction and primary investigation of antitumor activity of polysaccharides from the volva of Dictyophora rubrovolvata — Research Paper | ScholarLens