Progress on Phage Antibody Library Technology

Phage antibody library technology includes, amplify the full set variable region gene of antibody by PCR,connect the DNA fragments of antibody molecule with phage coat protein gene PⅢ or PⅧ,and then let the fusion protein be expressed on phage surface.Specific antibody is enriched and screened by adsorbing,eluting and amplification.This technology connects genotype with phenotype,which becomes a kind of high-performance scanning system.It unifies the ability which can identify antigen and phage's amplification and simulates the course of antibody production.In the following essay,the elements,construction,screening and application of phage library technology were summarized.