The Expression of SPmRNA in Rats with Immunesuppression and the Role of Acupuncture Intervention

Objective:To observe the immune suppression model of the expression of rat hypothalamus SPmRNA and different Acupuncture SPmRNA expression changes after the intervention.Methods:25 healthy SD rats Were randomly divided into normal control group(A group),model control group(B group),the model plus acupuncture group(C group),the model plus body acupuncture group(D group),the model plus acupuncture and body acupuncture group(E group).Immune suppression model prepared with cyclophosphamide injection,select the head points(Baihui and side two 0.5-inch acupuncture),body points(Zusanli and Guanyuan),head plus body to acupuncture,the time is 20 minutes,every 5 minutes twist pin.Using RT-PCR method to detect the brain tissue of rats SPmRNA expression situation.Data for each group mean±standard deviation indicated that the difference between the groups using analysis of variance(P0.05),significant difference as a standard.Results :The results show that the B group hypothalamus SPmRNA content was significantly decreased compared with the A group,there was significant difference(P0.05);after acupuncture treatment,C group and D group the expression of rat hypothalamus SPmRNA significantly decreased with the A group and B group significant difference(P0.05);E group expression in rat hypothalamic SPmRNA significantly increased compared with the B group,there was significant difference(P0.05);The E group was the best.Conclusion: Immune suppression SPmRNA expression in rat hypothalamus decreased significantly,head acupuncture plus body acupuncture points with acupuncture method can significantly increase the immune suppression SPmRNA expression in rat hypothalamus,enhance the body's immune function,whice is superior to body acupuncture,and acupuncture group.This mechanism may be immune suppression in rats by increasing the expression of hypothalamic SPmRNA by neurohumoral way to act on the peripheral tissue lymphoid tissue and cells,so that the low immune function have been restored to achieve.The experiment Provide the basis for acupuncture treatment of disease of low immune.