Effects of LPS stimulation on phenotype and functions of 1, 25(OH)_2D_3-treated dendritic cells

This study designed to investigate the effects of LPS stimulation on phenotype and functions of 1,25-dihydroxyvitamin D3(1, 25(OH)2D3)-treated dendritic cells(DC) in vitro. Firstly, 1, 25(OH)2D3-treated DC(10-8mol/L) were stimulated by LPS for 24 hours. Then the morphology of DC was observed by inverted microscopy; the expression of co-stimulatory molecules(CD80, CD86, and CD40) and MHC class Ⅱ was determined by flow cytometry; the levels of IL-12p70 and IL-10 were analyzed by ELISA. Furthermore, the capability of stimulating allogeneic CD4+T cell proliferation was measured by MLR(mixed lymphocyte reaction).1, 25(OH)2D3-treated DC stimulated by LPS exhibited features of inmature DC with high expression of costimulatory molecules CD86 and MHC class Ⅱ, inhibiting the secretion of IL-12p70, enhancing the secretion of IL-10, and suppressing allogeneic CD4+T cell proliferation. Results in this study indicate that 1, 25(OH)2D3-treated DC have the character of tolerogenic DCs, which can inhibit LPS-drived proliferation of CD4+T cells, which should be a foundation for study of 1, 25(OH)2D3-treated DCs inducing immunotolerance and the treatment of autoimmune disease such as asthma.