Effects of NF-κB “decoy” oligodeoxynucleotides on IL-10 expression in LPS-induced mouse macrophages

AIM: To study the effect of NF-κB “decoy”oligodeoxynucleotides on IL-10 expression in LPS-induced mouse macrophages. METHODS: Mouse macrophage cell line J774 1 cells were cultured with LPS and liposome-mediated oligodeoxynucleotides, and the level of IL-10 were measured in culture medium by enzyme linked immunosorbent assay. RNA was extracted from macrophages, and the mRNA expression of IL-10 were observed by RT-PCR. RESULTS: NF-κB “decoy”oligodeoxynucleotides did not inhibit the expression of IL-10 in LPS-induced macrophage, and did not reduce generation of IL-10. CONCLUSION: NF-κB decoy ODNs did not depress the mRNA expression of IL-10. NF-κB may not be the critical transcription factor in transduction mechanism of IL-10.