Study on inhibitory effects and mechanism of ardisiacrispin (A+B) on human promyeloleukemic HL-60 cells

Aim To study the inhibitory effect of ardisiacrispin(A+B) on human promyeloleukemic HL-60 cells and the underlying mechanism.Methods Cell growth inhibition in vitro was evaluated with MTT as- say. Ardisiacrispin (A+B)-induced cell cycle block and apoptosis were investigated using flow cytometry assay. Apoptitic bodies were detected by PI staining. Western blot was applied to determine modification of certain protein related to apoptosis. Results Ardisiacrispin (A+B) displayed dose dependent growth inhibition ability on HL-60 cells with the IC50 value of 4.2 mg·L-1 for 48 h. Ardisiacrispin(A+B) dose-dependently arrested HL-60 cells at the S phase of the cell cycle in the range of 1.0～5.0 mg·L-1,cells at S phase in treated group was 1.26、1.46 and 1.71 times of control group for 6、24 and 48 h of incubauion,respectively. Moreover, ardisiacrispin (A+B) induced dose-dependent apoptosis of HL-60 cells in the concentration of 1.0～7.5 mg·L-1.Furthermore,the PARP cleavage was detectable in a dose-dependent manner,2.5 mg·L-1 ardisiacrispin (A+B) generated a 27.1% of lysis fraction for 24 h of incubation. Conclusion Ardisiacrispin(A+B) exhibits prominent abilities to inhibit the proliferation of HL-60 cells by blocking the cell cycle at S phase and inducing apoptosis.