Reseach of hydroxyapatite nanoparticles inducing apoptosis of macrophage and its relation to Hsp70

To explore whether HAp nanoparticles induce apoptosis of macrophages and its correlation to Hsp70.Induce THP-1 cell lines to become macrophage with PMA.Co-culture the macrophages with 0,20,100μg/ml HAp nanoparticles in medium for 24h.Collect the macrophages,and dye with PI and AnexinV, then observe and detect the rate of apoptosis of the macrophages;determine the expression level of Hsp70 by Western Blot.THP-1 cell lines can be induced to macrophages.Detection for apoptosis shows that: observing through fluorescence microscope,positive apoptosis signal of 20,100μg/ml HAp particles groups are stronger than that of control group;detecting by flow cytometry(FCM),apoptosis rate of 20,100μg/ml HAp particles groups are also higher than that of control group(P10.0001,P20.0001),moreover,the rate depends on the concentration of HAp nanoparticles(P3=0.025).Detection by Western Blot result shows the expression of Hsp70 of 20,100μg/ml HAp particles groups increase comparing to control group,and the level also depends on concentrations of HAp nanoparticles.