SSR Analysis on Genetic Diversity of Natural Populations of Pistacia chinensis Bunge

Simple sequence repeat(SSR) molecular marker systems were used to analyze the genetic diversity and genetic structure of 8 natural populations of Pistacia chinensis.Based on establishment of optimized PCR reaction system,nine pairs of SSR primers were selected from the primers which were designed for Pistacia vera L.Totally,43 alleles at average of 4.78 for per locus were observed in the 8 populations and the average of polymorphic locus percentage(P) was 90.28%.The average of effective allele number per locus was 2.08,and the average of expected heterozygosity was 0.472,which showed the total genetic diversity of the populations at a moderate level.According to effective allele number and expected heterozygosity,the genetic diversity of the 8 populations from high to low were AKTXSPHXLYLC LZSX.The proportion of genetic differentiation among the populations accounted for 0.319,which indicated that P.Chinensis's variation mainly resulted from variation among populations.The 8 populations were divided into three types according to genetic distance.