The Regeneration Effect of Erythropoietin on Renal Tubular Epithelial Cells Stimulated by Aristolochic Acid

Objective:To investigate the effect of Erythropoietin on the regeneration of renal tubular epithelial cells stimulated by aristolochic acid.Methods:LLC-PK1 cells were stimulated by different concentrations of AA(5、10、20 μg/ml)and EPO(5、10、20 U/ml)were added as prophylaxis.The media of control contained neither of the agents.In situ cells apoptosis were detected by TUNEL and the proportions of cell proliferation were assessed by immunohistological staining of PCNA.Results:The staining of TUNEL suggested that compared to the control group,the positive proportion of nuclear stimulated by AA 5μg/ml showed no difference(P0.05),but the positive proportion increased significantly in AA 10 μg/ml and 20 μg/ml groups as compared to the control(P0.05).10 U/ml and 20 U/ml EPO reduced the proportions of apoptosis induced by AA 10 μg/ml(P0.05).The immunohistochemical staining of PCNA demonstrated that the positive expression of cells was increased by the stimulation of AA5μg/ml and decreased by the 10 μg/ml and 20 μg/ml AA;the PCNA positive cells were gradually increased after the intervention of various dose of EPO compared to the AA 10 μg/ml group(P0.05).Conclusion:EPO could inhibit apoptosis and promote regeneration of LLC-PK1 cells stimulated by aristolochic acid,which maybe one of the mechanism of EPO protecting renal tubular cells from injury and the mechanism of action needs to be further researched.