Effects of sevoflurane postconditioning on lungs of rats with lipopolysaccharide-induced acute lung injury

Objective: To investigate the effect of sevoflurane postconditioning on lungs of rats with LPS-induced acute lung injury.Methods: Thirty rats were randomly divided into 5 groups(n=6): NS group,LPS group,NS(1 ml/kg) or LPS(5 mg/kg) was instilled in rats′ airway.Sevoflurane groups(S 0.5 h group,S 1 h group,S 2 h group): Four hours after LPS instillation,rats received sevoflurane inhalation(2.4%) respectively for thirty minutes,one hour or two hours.Six hours after NS or LPS instillation,all groups were exsanguinated.Histopathological examinations were performed for the lung specimens and concentration of TNF-α,IL-1β and IL-10 in BALF were measured.Results: Compared with the NS group,after LPS instillation,the alveolar structure was severely altered.Concentration of TNF-α,IL-1β and IL-10 in BALF were significantly increased(P0.01).Compared with the LPS group,in sevoflurane groups,the alveolar structure was improved.Concentration of TNF-α,IL-1β and IL-10 were reduced(P0.05).It was more effective to improve pathohistology and reduce TNF-α,IL-1β levels in S 1 h group and S 2 h group than S30min group.Conclusion: Sevoflurane postconditioning could attenuate lung inflammation in rats with lipopolysaccharide induced acute lung injury,it was more effective during longer inhalation time.