2007•Anhui nongye kexueRequires access
Study on the Trehalose Protection on Activity of Luciferase
Yan Wang
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Abstract
The purpose of this study was to investigate the trehalose protection of luciferase at freeze-thawing,refrigeration and room temperature.The result indicated that,trehalose had the obvious protective function to the enzyme activity of luciferase,especially at freeze-thawing.After 3 times of freeze-thawing,the remained enzyme activity of trehalose group was 51.8%, the remained enzyme activity of non-trehalose group was 19.1% and trehalose group was 2.71 times as large as non-trehalose group.The best activity concentration on trehalose was 0.1 mol/L.