Chitinases Induced from Gliocladium catenulatum HL-1-1 and Their Antagonistic Activity Against Plant Pathogenic Fungi

Induction and antagonistic activity of chitinases produced by Gliocladium catenulatum HL-1-1 were studied. Results showed that the isolate could produce chitinases under various culture conditions. Sclerotia powder could induce the isolate to produce much more chitinases. The optimum conditions to produce the enzyme were pH 4.5 at initial medium and to culture the isolate for 6 days. The chitinases were proved to inhibit the conidial germination of Fusarium nivale, Coniella diplodiella, Curvularia lunata and Alternaria alternata and the sclerotial germination of Sclerotinia sclerotiorum and Rhizoctonia solani obviously.