Effects of androgen receptor antisense RNA on the growth characteristics of human prostatic cancer cell LNCaP

Objective To investigate the effects of androgen receptor (AR) antisense RNA on the growth characteristics of LNCaP Methods LNCaP cells transfected with AR antisense RNA retroviral vector pL AR SN were designed as LNCaPas AR LNCaP cells containing empty vector pLXSN were designed as LNCaP Neo LNCaP and LNCaP Neo served as the controls After LNCaP and transfected LNCaP cells were cultured in media with synthetic androgen R1881, 17β estradiol and progesterone, respectively, their growth was determined by trypan blue staining and 3H thymidine incorporation Results Compared with that of LNCaP and LNCaPNeo at 1 nmol/L R1881, growth of LNCaP as AR was inhibited significantly ( P 0 05) There was no difference in cell proliferation between LNCaP and LNCaPNeo( P 0 05) 3H thymidine incorporation test showed that the DNA synthetic rate of LNCaP as AR was significantly higher than that of LNCaP Neo ( P 0 01) Microscopic observation found LNCaP and LNCaP Neo cells grew well, but living LNCaP as AR cells were rare and dead in large amount Conclusion AR antisense RNA can change the androgen dependent characteristics of LNCaP cells and can also inhibit the proliferative responses of human prostatic cancer cell LNCaP to androgen