2007•Zhongguo shengwuzhipinxue zazhiRequires access
Serum-free Culture of Vero Cells and Influenza Virus on Microcarrier
WU Shu-jun
Open publisher page 0 citations
Abstract
Objective To explore the condition for serum-free culture of Vero cells and influenza virus on microcarrier.Methods Culture Vero cells and influenza virus in serum-free medium by using microcarriers in stirring flask.Observe the growth and adsorption of Vero cells and determine the hemagglutination titer of virus.Results Vero cells grew well on Cytodex 3 microcarrier.The optimal seeding density of Vero cells was 10 cells per bead.The hemagglutination titer of virus reached the maximum 72 h after inoculateion at a MOI of 0.005 or 0.010 CCID_(50)/cell.Conclusion In the absence of serum,Vero cells grew well on Cytodex microcarrier,and influenza virus was successfully cultured in the cells.