Effects of hPOT1 overexpression on cell cycle and the apoptosis of HeLa cells

ObjectiveTo observe the effects of hPOT1 overexpression on cell cycle and apoptosis of HeLa cells. MethodsBy using an eukaryotic expression plasmid of pcDNA-hPOT1, liposomal mediates transient transfection of HeLa cells. The expressing effects of foreign genes were detected by RT-PCR and EMSA method. The cell cycles were analyzed by flow cytometry. And cell apoptosis was detected by Hoechst 33342 fluorescent staining. ResultsAfter 48 hours of the pcDNA3-hPOT1 transfected into HeLa cells, mRNA and protein analysis showed that hPOT1 could be efficiently expressed. Cell cycle in HeLa cells was blocked in S phase, and the overexpression of hPOT1 didnt show the effects on apoptosis. ConclusionThose results proved that hPOT1 functioned in the regulation of cell cycle in eukaryotic cells but not in cell apoptosis.