Rapid defection Aeromonas hydrophila and Edwardsiella by multiplex polymerase chain reaction

The multiplex polymerase chain reaction(PCR) was developed and optimized to detect and identify Aeromonas hydrophila and Edwardsiella simultaneously.Two pairs of specific primers XZAH3,XZAH4 and XZE7b,XZE8 were designed according to the sequences of 16S rDNA of Aeromonas hydrophila and Edwardsiella.The results showed that only 361pb-long DNA fragment for the Edwardsiella strains was amplified and the 576pb-long DNA fragments for the Aeromonas hydrophila were amplified by the multiplex PCR with these two pairs of primers,but the other fish pathogenic viruses and bacteria were not amplified.As little as 10 pg DNA of Aeromonas hydrophila and Edwardsiella could be detected by the multiplex PCR.