2013•Chinese Journal of Injury Repair and Wound HealingRequires access
Protective effect of Nω-nitro-L-arginine in rats lungs with smoke inhalation injury
Yuan Zhi-nan
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Abstract
Objective To explore the protective effects of Nω-nitro-L-arginine (L-NNA) in lungs of rats with smoke inhalation injury.Methods Sixty male Sprague-Dawley rats were randomly divided into 4 groups,6 in blank group.The remaining rats were divided into saline group,low dose group and high dose group,and each group set 6 h,24 h,48 h three time points,6 rats in each time point.The rats without any treatment were put in blank group,the remaining three groups of rats were making models of smokeinhalation injury.Saline group,low dose group and high dose group after injury were injected with equivalent saline,the concentration of 10 mg/kg L-NNA and the concentration of 20 mg/kg L-NNA.Each rat took blood 5 mL after 30 minutes of injection,centrifuged and tested interleukin 6 (IL-6 ) ,interleukin-10 (IL-10) ,tumor necrosis factor α (TNF-α) content in serum,and tested malondialdehyde (MDA) , myeloperoxidase (MPO) and superoxide dismutase (SOD) content in lung,examined the lung tissue under microscope.Results Low dose group 48 h TNF-α (179.51 ± 8.67) ng/L vs (231.39 ± 9.98) ng/L, IL-6 (289.01 ± 31.84) pg/mL vs (398.67 ± 32.93) pg/mL,MDA (4.37±0.62) nmol/mg vs (4.41 ± 0.35) nmol/mg,MPO (41.39 ± 1.49) U/g vs (50.18 ± 1.72) U/g decreased compared to the saline group (P0.01) ,SOD (93.82 ± 5.69) U/mg vs (88.46 ± 5.48) U/mg and IL-10 (110.33 ± 4.21) ng/L vs (108.85 ± 5.47) ng/L decreased compared to the saline group (P0.01) .The discrepancy between high dose group and saline group was more statistically significant than low dose group.Pathological section display that there were fewer inflammatory cells and slightly reduced alveolar collapse in lung tissue of rat in low dose group and high dose group compared with saline group at 6 h after injury,and more significant improvements in high dose group than in the low dose group.Conclusion L-NNA can reduce inflammation and chemical reaction in the lung tissue of rats after inhaled injury,so as to improve lung injury level.