Experimental Study of Radiosensitivity of Human Glioma Cell Lines

Objective To investigate the intrinsic radiosensitivity of 11 glioma cell lines in vitro. Methods Eleven glioma cell lines, i.e. T98G, SF295, Skmg-1, Skmg-4, MGR-1, MGR-2, MGR-3, UWR-7, SF767, SHG-44 and uw28 were used in this study. The intrinsic radiosensitivity parameters of 11 human glioma cell lines were determined in vitro by clonogenic assay after different doses of X-irradiation (0～8Gy). The survival curves were generated and mathematically analysed by using the L-Q model to obtain the radiosensitivity parameters, alpha, beta, SF_2 (the surviving fraction at 2 Gy) and SF-8 (the surviving fraction at 8 Gy). Results There were great differences in the radiosensitivity among 11 glioma cell lines. For 11 glioma cell lines, the planting efficiency varied from 1.61% to 40.89%. The mean SF_2 was 0.44±0.10 with range of 0.32 to 0.59 and the mean SF_8 was 0.42×10~(-2)±1.1×10~(-2) with range of 0.00 to 3.71×10~(-2). Conclusion The intrinsic radiosensitivity varies in different human glioma cells lines.