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Application and Activity Assay of Co-expressed Organophosphorus Hydrolase and Carboxylesterase(B1) Produced by Genetic Engineering Bacteria

Qiao Chuan-ling

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Abstract

Carboxylesterase and organophosphorus hydrolase (OPH) are capable of hydrolyzing and detoxifying different pesticides. In order to get the widest substrate spectrum organophosphorus hydrolase and carboxylesterase (B1) were co-expressed in one genetic engineering bacteria. The activity of OPH was assayed by orthogonal test, and the optimum condition were substrate concentration 2×10-4mol/L, temperature 25℃, reaction time 10min and pH8.0. In the detoxification of parathion on mosquitoes, 95% of the mosquitoes can be survived. And it has been used in biosensor for pesticide detection.

About this research paper

What this paper is about

Carboxylesterase and organophosphorus hydrolase (OPH) are capable of hydrolyzing and detoxifying different pesticides. In order to get the widest substrate spectrum organophosphorus hydrolase and carboxylesterase (B1) were co-expressed in one genetic engineering bacteria. The activity of OPH was assayed by orthogonal test, and the optimum condition were substrate concentration 2×10-4mol/L, temperature 25℃, reaction time 10min and pH8.0. In the detoxification of parathion on mosquitoes, 95% of the mosquitoes can be survived. And it has been used in biosensor for pesticide detection.

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Available abstract

Carboxylesterase and organophosphorus hydrolase (OPH) are capable of hydrolyzing and detoxifying different pesticides. In order to get the widest substrate spectrum organophosphorus hydrolase and carboxylesterase (B1) were co-expressed in one genetic engineering bacteria. The activity of OPH was assayed by orthogonal test, and the optimum condition were substrate concentration 2×10-4mol/L, temperature 25℃, reaction time 10min and pH8.0. In the detoxification of parathion on mosquitoes, 95% of the mosquitoes can be survived. And it has been used in biosensor for pesticide detection.

Key concepts: Carboxylesterase, Hydrolase, Parathion, Substrate (aquarium), Chemistry, Enzyme, Bacteria, Pesticide

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Application and Activity Assay of Co-expressed Organophosphorus Hydrolase and Carboxylesterase(B1) Produced by Genetic Engineering Bacteria — Research Paper | ScholarLens