Analysis on Changes of Key Nonvolatile Organic Acids in Tobacco Leaves during Its Growth by Gas Chromatography-Mass Spectrometry

[Objective] The changes of nonvolatile organic acids in tobacco leaves during its growth were studied.[Method] By using the coupling technique of N-methyl-N-trimethylsilytrifluoroacetamide(MSTFA) derivatization-gas-chromatography-mass spectrometry,11 kinds of key were analyzed through the combination of standard sample and mass spectrum.[Result]The determination on analyzing index of the method for 6 nonvolatile organic acids with the standard sample showed a good average recovery,with the recovery of 85.4%-102.1% and the relative standard deviations(RSD) less than 7.0%.[Conclusion] The method had the simple and convenient,rapid,accurate and other characteristics and was applicable to the determination of the nonvolatile organic acids in tobacco leaves.When the method was applied to determine the changes of nonvolatile organic acids in tobacco leaves during its growth,the change curves of 11 key nonvolatile organic acids in tobacco leaves during its growth was obtained.