Experimental study of myocardial protection with retrograde coronary sinus perfusion in beating heart

Objectives To evaluate the myocardial protective effects of retrograde coronary sinus perfusion in beating heart.Methods Isolated rabbit working heart models were used in this study.Fourty 3 to 4 months rabbits were rondomly divided into 2 groups: group A (beating heart with continuous 32?℃ warm blood perfusion,n=10),group B (continuous 32?℃ warm blood cardioplegia arrest,n=10 ).Left ventricular systolic pressure (LVSP),left ventricular end diastolic pressure ( LVEDP) were measured.Creatine kinase (CK) leakage from blood samples in coronary sinus,ATP and malondialdehyde(MDA)in left and right ventricles were assessed 120?min after continuous perfusion through retrograde coronary sinus.Results Recovery rate of LVSP,LVEDP in the group A were 91.69% and 111.31%.ATP was (11.45±0.91)?nmol/gdw in the groups,higher than in the group B (9.98±0.85)?nmol/gdw,P0.05).The levels of MDA(2.34±0.57?nmol/mg)and CK leakage [33.69±3.09?IU/(5?min.gdw)] were lower singnificantly in the group A than in the group B (P0.05).There was no significant difference in the above parameters between the left and right ventricles in the group A (P 0.05). Conclusion Retrograde coronary sinus perfusion in beating heart has good protective effects on both left and right ventricles and is a safe and effective method for myocardia.