The influence of glybenzcyclamide on cardiac ischemic preconditioning against myocardial ischemia-reperfusion injury induced by ATP-sensitive potassium channels

Objective To study the effect of glybenzcyclamide (GLC) on cardiac ischemic preconditioning (IP) against myocardial ischemia-reperfusion (IR) injury induced by ATP-sensitive potassium channels (KATP).Methods 44 rats were randomly divided into IR preconditioning (IPC) group, GLC group, GLC-preconditioning (G-P) group and control group. The preconditioning protocol consisted of 3 cycles of 10 min I/R.The rats were subjected to 30 min of local ischemia followed by 60 min of reperfusion. The infarct size was measured by nitroblue tetrazolium and red tetrazolium staining and signed by percentage of necrosis zone in ischemia zone.The cardiac ventricular arrhythmia was recorded by Ⅱ lead.Results IPC resulted in marked reduction in infarct size,which was completely abolished by glybenzcyclamide. IPC also reduced I/R induced ventricular arrhythmias,which couldn′t be blocked by glybenzcyclamide.Conclusions Glybenzcyclamide could influence the protective role of KATP induced IPC myocardial preservation.