Effects of Copper on Activities of Antioxidant Enzymes and Total Antioxidative Competence in Hepatopancreas of Cyprinus Carpio

Mirror Cyprinus carpio were exposed to different concentrations of copper,0.01,0.05,0.10,0.30,0.50,0.70 and1.00 mg·L-1,the activities of three antioxidant enzymes,superoxide dismutase(SOD),catalase(CAT) and glutathion peroxidase(GPx),and total antioxidative competence(T-AOC) in hepatopancreas were determined after 1,3 ,5 and 7 days of exposure in order to study the effects of copper on activities of antioxidant enzymes and T-AOC in hepatopancreas of Cyprinus carpio.The results showed that:when fish were exposed to the concentrations of 0.01 mg·L-1,the activities of SOD,CAT and GPx and T-AOC were higher than those of the control respectively(P0.05),the activities of GPx reached a peak,and T-AOC was significantly higher than that of the control after 3 days(P0.05).When fish were exposed to the concentrations of 0.05 mg·L-1,the activities of SOD and CAT and T-AOC(P0.05) reached the peak,and the activities of SOD and CAT were significantly higher than those of the control after 3 days of exposure(P0.05),and the activities of GPx were higher after 1 day,lower after 3 days and significantly lower after 5 days compared with those of the control.When fish were exposed to the concentrations of 0.10 mg·L-1,the activities of SOD were significantly lower than those of the control after 3 days(P0.05),and the activities of CAT and GPx and T-AOC were significantly lower(P0.05) compared with those of the control;When fish were exposed to the concentrations of 0.30 mg·L-1 or higher,the activities of SOD,CAT and GPx and the T-AOC were significantly inhibited(P0.05).These results indicated that:the activities of SOD,CAT and GPx and T-AOC in hepatopancreas of Cyprinus carpio might act as indicative functions for the copper contamination(the activities of GPx were the most sensitive).They could be used as a biomarker in evaluating heavy metal contamination.