ScholarLens Back to search
2010•China Animal Husbandry & Veterinary MedicineRequires access

Construction and Analysis of a Full-length cDNA Library of Ascosphaera apis

Xuefeng Zhang

Open publisher page 0 citations

Abstract

In this study,the total RNA of Ascosphaera apis was extracted by using TRIzol reagent.The Oligo reverse transcriptase was used to synthesize and anchor the first strand Cdna.Following the production of the first reverse transciption,we synthesize the second Cdna.The production of cDNA was separated by classes and packed outside,then we could get the cDNA original library of Ascosphaera apis.The unamplified cDNA library contaied 1.6×106 PFU/mL independent clones.The titer of the amplified library was 1.5×109 PFU/mL.Taking suitable amplified library to dilute and litter the flat,picking single plaques and then using common primer M13± to amplify,the recombination clones with an average insert size of 0.5 to 2 kb was higher than 90%.The construction of this library will provide a platform for the research of invasion mechanism and prevention.

About this research paper

What this paper is about

In this study,the total RNA of Ascosphaera apis was extracted by using TRIzol reagent.The Oligo reverse transcriptase was used to synthesize and anchor the first strand Cdna.Following the production of the first reverse transciption,we synthesize the second Cdna.The production of cDNA was separated by classes and packed outside,then we could get the cDNA original library of Ascosphaera apis.The unamplified cDNA library contaied 1.6×106 PFU/mL independent clones.The titer of the amplified library was 1.5×109 PFU/mL.Taking suitable amplified library to dilute and litter the flat,picking single plaques and then using common primer M13± to amplify,the recombination clones with an average insert size of 0.5 to 2 kb was higher than 90%.The construction of this library will provide a platform for the research of invasion mechanism and prevention.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

In this study,the total RNA of Ascosphaera apis was extracted by using TRIzol reagent.The Oligo reverse transcriptase was used to synthesize and anchor the first strand Cdna.Following the production of the first reverse transciption,we synthesize the second Cdna.The production of cDNA was separated by classes and packed outside,then we could get the cDNA original library of Ascosphaera apis.The unamplified cDNA library contaied 1.6×106 PFU/mL independent clones.The titer of the amplified library was 1.5×109 PFU/mL.Taking suitable amplified library to dilute and litter the flat,picking single plaques and then using common primer M13± to amplify,the recombination clones with an average insert size of 0.5 to 2 kb was higher than 90%.The construction of this library will provide a platform for the research of invasion mechanism and prevention.

Key concepts: Trizol, Complementary DNA, cDNA library, Insert (composites), Reverse transcriptase, Biology, Titer, Primer (cosmetics)

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Construction and Analysis of a Full-length cDNA Library of Ascosphaera apis — Research Paper | ScholarLens