2010Applied Chemical IndustryRequires access

The antagonism of forbearing low temperature Trichoderma against Botrytis cinerea

Zhihua Jia

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Abstract

The antagonism of the forbearing low temperature Trichoderma against Botrytis cinerea was tested in vitro.On dual culture at 4 ℃,they grew fast,formed the obvious inhibitory strip when meeting B.cinerea,and induced it to produced the compact spore layer and sclerotiet Botrytis hyphae were coiled,penetrated,lysed and broken by XJTR-165.The extracellular antibiotic substance secreted by the Trichoderma could inhabit spore-sprouting to the highest amount of 30% and hyphal growth with the inhabiting ring from 0.4 to 2 cm.Trichodruma could produced the extracellular chitinase(up to 0,24 U/mL by RT-165) when induced by Botrytis hyphal fragment of 20%,but could not or a little in PDA culture.

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What this paper is about

The antagonism of the forbearing low temperature Trichoderma against Botrytis cinerea was tested in vitro.On dual culture at 4 ℃,they grew fast,formed the obvious inhibitory strip when meeting B.cinerea,and induced it to produced the compact spore layer and sclerotiet Botrytis hyphae were coiled,penetrated,lysed and broken by XJTR-165.The extracellular antibiotic substance secreted by the Trichoderma could inhabit spore-sprouting to the highest amount of 30% and hyphal growth with the inhabiting ring from 0.4 to 2 cm.Trichodruma could produced the extracellular chitinase(up to 0,24 U/mL by RT-165) when induced by Botrytis hyphal fragment of 20%,but could not or a little in PDA culture.

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Available abstract

The antagonism of the forbearing low temperature Trichoderma against Botrytis cinerea was tested in vitro.On dual culture at 4 ℃,they grew fast,formed the obvious inhibitory strip when meeting B.cinerea,and induced it to produced the compact spore layer and sclerotiet Botrytis hyphae were coiled,penetrated,lysed and broken by XJTR-165.The extracellular antibiotic substance secreted by the Trichoderma could inhabit spore-sprouting to the highest amount of 30% and hyphal growth with the inhabiting ring from 0.4 to 2 cm.Trichodruma could produced the extracellular chitinase(up to 0,24 U/mL by RT-165) when induced by Botrytis hyphal fragment of 20%,but could not or a little in PDA culture.

Key concepts: Botrytis cinerea, Antagonism, Hypha, Trichoderma, Botrytis, Spore, Chitinase, Trichoderma harzianum

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