Comparison of free intracellular Ca~(2+)in hippocampus cells at different time points after focal cerebral ischemia/reperfusion in rat

Objective To study changes of free intracellular Ca~(2+) after focal cerebral ischemia/ reperfusion on rat and the effect of meglumine adenosine cyclosphosp(MAC) pretreatment on them.Methods Sixty Sprague-Dawley rats were randomly divided into four groups:normal group (5) ,sham operated group(5) ,ischemia/reperfusion group(reperfusion group) ,MAC pretreatment group(MAC group).Reperfusion group and MAC group were further divided into 5 groups at 2, 24,48,72 h and on d 7 after reperfusion,5 rats at each time point.The acute focal cerebral ischemia /reperfusion models were established with suture embolus method as described by Nagasawa. The content of free intracellular Ca~(2+) in hippocampus cells was detected by flow cytometry.Results Compared with sham operated group,in reperfusion group,the content of free intracellular Ca~(2+) of hippocampus cells began to increase at 2 h,peaked at 24,48 h (P 0.01),began to decrease at 72 h and further decreased on d 7,however,it was still higher than normal level.Compared with reperfusion group,there was no statistically significant difference of intracellular Ca~(2+) at 2 h,but it significantly decreased at 24,48,72 h in MAC group (P0.01).Conclusions Intracellular Ca~(2+) elevation in hippocampus cells after ischemia/reperfusion is one of pathologic mechanisms in ischemia/reperfusion injury.Pretreatment with MAC can inhibit this elevation significantly.