Screening and Identification of Antagonistic Bacteria against Tomato Bacterial Wilt

[Objective] Antagonistic bacteria against tomato bacterial wilt were screened out to provide reference for the control of tomato bacterial wilt. [Method]Antagonistic bacteria against tomato bacterial wilt were obtained from the soil samples of tomato fields,and identified through the observation of morphological characteristics,detection of physiology-biochemistry and 16 S r DNA analysis,and the control efficiency was verified by pot experiment. [Result] A total number of 104 strains bacteria were isolated from Zhangjiajie City,Hunan Province on Nutrient Agar Plate,and there were six strains against tomato bacterial wilt. Among them,one bacteria was found to be strong antagonistic effect against the Ralstonia solanacearum,with the inhibition zone of 7. 2 mm,and the control efficiency of fermentation liquid reached 96. 4% at greenhouse experiments. The strain 88 was identified as Burkholderia sp.. [Conclusion]The strain 88 has broad application prospects in controlling potato soil borne disease.