A comparison of a rat hind-limb ischemia model established by ligation and embolization

Objective To explore an effective method for establishing a hind-limb ischemia model in rats.Methods Wistar rat hind-limb ischemia models were established by three methods: ligation of the femoral artery,ligation of the common iliac artery,embolization of the femoral artery with fine particles of the common bletilla tuber(FP-CBT).For 1 to 21 days after operation,the ischemia and function were observed.Results ① Temporal limp happened in the operated hind-limbs after ligating the femoral artery or the common iliac artery,and no pathological changes were found in the tissue sections;② At 3 to 21 days after embolizing the femoral artery by FP-CBT,severe ischemia occurred in the operated hind-limbs,such as that sustained by limping,skin necrosis or ulcers,and muscle fibers necrosis which was recognized by HE staining.Conclusion Hindlimb ischemia can not be produced by ligating the artery.However,it can be well induced by embolizing the femoral artery with FP-CBT.