Changes in Peripheral Blood Lymphocyte Aubsets and Regulatory T Cells in Idiopathic Thrombocytopenic Purpura Patients and the Clinical Significance

Objective To study the changes and clinical significance of lymphocyte subsets and regulatory T cells in peripheral blood of patients with idiopathic thrombocytopenic purpura(ITP).Methods Peripheral blood lymphocyte subsets and regulatory T cells of 40 ITP patients and 40 normal controls were measured with flow cytometry.Results Compared with the normal group,the percentage of CD3+ T lymphocyte,CD4+ T lymphocyte and CD16+ /56+ NK cell and ratio of CD4+ /CD8+ decreased markedly in ITP group(P 0.01),while the percentage of CD8+ T lymphocyte increased slightly(P 0.05) and CD19+ B lymphocyte increased significantly(P 0.01).In ITP patients,the proportion of CD4+ CD25+ T cells in CD4+ T cells was higher than that of the control group(P 0.05) and the proportion of CD4+ CD25high T cells was slightly higher(P 0.05),while the proportion of CD4+ CD25+ Foxp3+ regulatory T cells was lower(P 0.05).Conclusion Cellular immune function in patients with ITP is obviously abnormal and the decreased proportion of CD4+ CD25+ Foxp3+ regulatory T cells may be related to the immune pathogenesis of ITP.Detection of lymphocyte subsets and regulatory T cells is of some clinical value in evaluation of therapeutic effect and prognosis of ITP.