Cloning of F1 Gene of Newcastle Disease Virus F48E9 Strain and Its Expression in Escherichia coli

According to the encoding sequence of the NDV F48E9 strain F1 protein and the multiple cloning sites characteristic of prokaryotic expression vector pET-28a in GenBank,appropriate primers specific to NDV F1 gene were designed.The target DNA fragments were obtained by PCR amplification.Then the F1 gene was subcloned into a prokaryotic expression vector pET-28a and the recombinant was designated as pET-F1.The target protein was expressed in E.coli Rosetta(DE3) induced with IPTG.The results of SDS-PAGE and Western-blotting indicated that the F1 gene was expressed from pET-F1 induced by IPTG at high level,and the expressed fusion protein was 31ku approximately in molecular mass.The result provided fundamental hases and materials for the further investigation on immunogenicity and molecular biological function of F1 of NDV.