Cloning of Sucrose Synthase Gene from Sugarcane(Saccharum officenarum L.)

Sucrose synthase 2 (SuSy2) is a key enzyme regulating sucrose metabolism in higher plants, which promotes sucrose into various metabolic pathways. The degenerated oligonucleotides to highly conserved region of SuSy2 were used to prime the amplification of specific fragment by PCR in samples of sugarcane DNA. One fragment of about 7.5 kb was cloned and its complete nucleotide sequence was determined. Its nucleotide sequence and the protein from its coding region was 99.5% and 100% identical to the deduced proteins from the sugarcane SuSy2 cDNA. The sequence also included about 1.9 kb upstream promoter regulation,sixteen extrons, fifteen introns, 3' untranslatded region, and the open read frame coded 802 amino acids. The aminoacid sequence had a conservative sucrose metabolism key enzyme family Ser/Thr bit point.All that laid the foundation for its transcriptional expression regulation mechanism.