Influence of siRNA expression vector on cervical carcinoma Caski cell apoptosis and proliferation

Objective To observe the effect of HPV16 E6 specific expression vector on cell proliferation and apoptosis in cervical carcinoma Caski cells.Methods HPV16 E6 siRNA expression vector and empty expression vector were transfected into Caski cells by liposome.The effects on E6 mRNA,E6 protein expression and cell apoptosis were examined respectively by real-time RT-PCR and FCM;Cell growth rate and cloning efficiency were examined by MTT and colony forming assay.Results HPV16 E6 siRNA expression vector significantly inhibited the expression levels of E6 mRNA and E6 protein,the inhibition rates being 89.5%and 98.1%respectively.Conclusion HPV16 E6 specific siRNA expression vector could specifically and efficiently inhibit the expression of E6 gene.It could induce apoptosis more effectively and inhibit cell proliferation in cervical carcinoma Caski cells.