Cloning and prokaryotic expression of bovine myostatin gene

In order to express the functional sequence of bovine myostatin gene in the prokaryotic system, a pair of primers was designed according to the myostatin cDNA sequence and optimized with restriction sites for two restriction endonucleases EcoR I and Xho I . The functional fragment was amplified from bovine total RNA using RT-PCR. The cloning plasmid and pET-28a vector were digested, retrieved and sequenced,respectively.Double digestion with EcoR I and Xho I,PCR identification and sequence analysis showed that both the cloning vector and the prokaryotic expression vector for the functional fragment of the myostatin gene were constructed successfully. Positive clones were selected and induced by IPTG, and the expressed product of the bovine functional fragment was obtained.