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2012•Journal of Qiqihar UniversityRequires access

Optimization of detection of clenbuterol residue in pork by solid phase Extraction-HPLC method

Wei Han

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Abstract

In this paper,solid-phase extraction—RP-HPLC was applied for the detection of clenbuterol residue in pork and both the pre-treatment method and chromatographic conditions were optimized.For the pre-treatment of the meat samples,the best ultrasonic extraction time and elution speed of the extracts on solid phase were 30 minutes and 2d/s,respectively.Then the purified extracts was analyzed by RP-HPLC on a ODS C-18 column(4.6μm×l50 mm)using 0.01mol/L of solium dihydrogen phosphate solution-methanol(65∶35)as mobile phase at a flow rate of 1mL/min and a UV detector at 243nm.This method for clenbuterol residue detection in pork had high accuracy,residual recovery,and good reproducibility.

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What this paper is about

In this paper,solid-phase extraction—RP-HPLC was applied for the detection of clenbuterol residue in pork and both the pre-treatment method and chromatographic conditions were optimized.For the pre-treatment of the meat samples,the best ultrasonic extraction time and elution speed of the extracts on solid phase were 30 minutes and 2d/s,respectively.Then the purified extracts was analyzed by RP-HPLC on a ODS C-18 column(4.6μm×l50 mm)using 0.01mol/L of solium dihydrogen phosphate solution-methanol(65∶35)as mobile phase at a flow rate of 1mL/min and a UV detector at 243nm.This method for clenbuterol residue detection in pork had high accuracy,residual recovery,and good reproducibility.

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Available abstract

In this paper,solid-phase extraction—RP-HPLC was applied for the detection of clenbuterol residue in pork and both the pre-treatment method and chromatographic conditions were optimized.For the pre-treatment of the meat samples,the best ultrasonic extraction time and elution speed of the extracts on solid phase were 30 minutes and 2d/s,respectively.Then the purified extracts was analyzed by RP-HPLC on a ODS C-18 column(4.6μm×l50 mm)using 0.01mol/L of solium dihydrogen phosphate solution-methanol(65∶35)as mobile phase at a flow rate of 1mL/min and a UV detector at 243nm.This method for clenbuterol residue detection in pork had high accuracy,residual recovery,and good reproducibility.

Key concepts: Chromatography, Clenbuterol, Residue (chemistry), High-performance liquid chromatography, Solid phase extraction, Chemistry, Reproducibility, Elution

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