Haemophilus isolation and resistance rate during 2000～2001

Objective Investigate isolation rate and analyze resistance of Haemophilus mfluenzae and parainfluenzae during 2000～2001 in our hospital. Methods Samples were inoculated in the Chocolatc Haemophilus Agar, the strains were identified and β-lactamase and resistance test were carried out. Results In 974 samples from respiratory tract we found 8 strains of Haemophilus influcnzae, with β-lactamase(+) strains being 0, and 13 strains of Haemophilus parainfluenzae β-lactamases 7.69 in 2000. In 845 samples we found 25 strains of Haemophilus influenzae, and with β lxctamase(+) strans 24 and Haemophilus parain fluenzae 120 strains with β-lactamase 17.5 and were found 1 strain β-lactamase negative in 2001. Conclusion Haemophilus is a very important infectious agent in respiratory tract, and the culture medium quality is a key factor to isolation rate of Haemophilus. In 2001 Haemophilus infuenzae β-lactamases(+) stain was 24, but it was unusual to find multi-resistance. Haemophilus parainfluenzae resistance is more complex than that of Haemophilus influenzae, it occurs by other mechanisms and is more difficult in terms of treatment. The correct use of antibiotics is the best way to decrease drug resistance.