PLK1 Gene Silencing Decreases Apoptosis of HeLa Cells

HeLa cell, one kind of Cervix carcinoma cell line, was used to investigate the effect of short hairpin RNA on PLK1 gene with high expression of PLK1. Short hairpin RNA targeting at Polo-like kinase 1 gene was designed and synthesized, which was transfected into RNAi expression vector with EGFP. PLK1 expression in HeLa cells was detected by RT-PCR and Western blotting respectively. Cells apoptosis were measured by flow cytometry, and proliferation was detected via MTT assay. pEGFP-H1, an RNAi expression vector, was successfully constructed. Transfected with shRNA, PLK1 expression in HeLa cells was decreased to 30%. Compared with control group and mock-transfected group, apoptosis of HeLa cells transfected with shRNA was remarkably increased(P0.05), but their proliferation activity was reduced(P0.05). The RNAi expression vector constructed was more conveniently to detect the genes transfection and did not affect its transcription.PLK1 gene silence can obviously increase apoptosis of HeLa cells and inhibit their proliferation, which may be the new target and efficient way for tumor therapy in the future.