Study on the Relationship between ApoAI Gene Promoter Polymorphism and Coronary Heart Disease and Serum Lipid Levels

Objective:To investigate the relationship between Apo AI gene promoter polymorphism and coronary heart disease(CHD)and serum lipid levels in Chinese population.Methods:The restriction fragment polymorphisms(RFLP)at two MspI sites in the5'end at-75bp and+83bp of apolipoprotein AI gene were detected using PCR in240coronary heart disease patients and196healthy subjects from a population of Chinese Han nationality in Tianjin.Results:G/G genotype was the most frequency in CHD and control groups,but the frequency of A/A genotype in CHD group was significantly lower than that in control group(P0.05).No difference was found in the frequencies of rare A allele for-75bp and rare T allele for+83bp.In CHD group,male subjects with A/A genotype had a lower serum mean concentration of HDL compared with male subjects with genotype G/G(P0.05).Conclusion:MspI RFLP at-75bp of apo AI gene is associated with coronary heart disease in Chinese population,and the A/A genotype of apo AI gene is associated with the decrease of serum HDL level.